URINE FOR GCMS

Metabolomics is a method for studying the overall metabolic profile in biological fluids and tissues, with urine being a commonly used sample due to its rich content of metabolites. Gas chromatography–mass spectrometry (GC–MS) is a common analytical tool in metabolomics, and the pre-analytical steps involve urease pre-treatment and urine storage. Urease pre-treatment is necessary to remove interfering urea, and proper urine storage is crucial for preserving the metabolic profile. This study aims to assess the impact of urease pre-treatment, optimize its effectiveness, and examine short-term urine storage at −80 °C. Understanding these factors is vital for reliable and accurate metabolomic analyses in various medical and research settings.