WESTERN BLOT CONFIRMATION FOR HIV I & II

The Western Blot confirmation test for HIV I & II stands as a pivotal and sophisticated laboratory procedure utilized by Hypro Diagnostics to substantiate the presence of antibodies to the human immunodeficiency virus in an individual’s blood. This test assumes significance as a secondary diagnostic step, particularly following an initial reactive outcome from screening tests like enzyme immunoassays or rapid antibody tests. Leveraging precision, the Western Blot meticulously examines specific HIV proteins by separating them based on size and then transferring them onto a membrane. Key viral components such as the core protein (p24) and envelope proteins (gp41, gp120/160) are targeted. Upon applying the patient’s serum, antibodies to HIV prompt a binding reaction with the corresponding viral proteins on the membrane. A positive outcome in the Western Blot is deemed conclusive evidence of HIV infection. It’s important to emphasize that, while the Western Blot is highly specific, Hypro Diagnostics recognizes its role as a complementary rather than a standalone diagnostic tool due to its complexity and the potential for false positives. This approach aligns with established guidelines for HIV testing, making the Western Blot an integral component in Hypro Diagnostics’ diagnostic algorithm for HIV infection confirmation.